To 500L of the interacted sample, 100L of acridine orange (0.1% solution in distilled water) was added and incubated in dark for 10min. After 10min, the unbound stain was removed by centrifugation at 5000rpm for 10min. This washing was repeated thrice and the acridine orange stained cells were finally suspended in 500L sterile distilled water.
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